Clarke scored 11 goals in 15 appearances while on loan early in the 2012-13 season, and has been linked with a move away from Wolves this summer.
He only joined the Molineux club in January, with £750,000 spent to take him from Coventry, but he has found first-team opportunities limited.
He was linked with Crawley earlier this summer, and speculation has suggested a return to Glanford Park – especially if in-demand forward Sam Winnall moves on.
Wilcox insisted that talk of an approach from the Iron was wrong, and is not planning on a move.
‘We’ve not made an enquiry. Not at all,’ Wilcox told the Scunthorpe Telegraph.
‘Sometimes somebody puts something out there and from one story or tweet, people jump on it, put two and two together and next minute, Leon Clarke is coming to Scunthorpe.
‘That’s not the case. He’s been here before and I’ve worked with him here and at Sheffield Wednesday, but I’ve not heard anything to even suggest he is unhappy at Wolves.
‘Leon is a Championship player now and I’m sure he wants to play at that level.’